Isolation of Wheat Microsatellite Dna Fragments by Hybridization Selection
نویسندگان
چکیده
A procedure for selection of wheat genomic fragments containing various microsatellites is described. The procedure is based on the hybridization of single-stranded DNA fragments either to filter bound microsatellite DNA or to biotinylated oligonucleotides in solution. Hybridized strands are isolated, amplified and cloned. Results presented show that enrichment for (CA)n/(TG)n microsatellites depends on hybridization conditions and on amplification protocol. Enrichment can be significantly improved by performing several hybridization selection rounds. The sequences of two wheat microsatellite clones are presented. The method is simple, saves time and may be used for microsatellite fragment isolation from plant DNA for genetic, evolutionary or population studies.
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